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Cells were imaged as described above.
In only a few cells a diffuse punctate pattern might be observed after fixation, as an example, for that construct GFP Sec16B (109–.
Again, as the control, HeLa cells transfected with pEGFP C2 have been.
GFP Sec16B is knocked down efficiently with siRNAs except Sec16B UTR which only targets the endogenous Sec16B.
1% of input and unbound fraction in addition to 20% on the beads were separated by SDS PAGE.
Transmission electron microscopy imaging (TEM)Cells were grown on Thermanox cover slips (Ted Pella Inc.
235) and GFP Sec16B (109–.
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